human b ngf antibody  (R&D Systems)

Journal: Oncogene

Article Title: Nerve growth factor overexpression and autocrine loop in breast cancer cells.

doi: 10.1038/sj.onc.1206805

Figure Lengend Snippet: Figure 2 Immunocytological localization of NGF in human breast cancer cells. (a) Immunocytochemistry of NGF in normal (NBEC) and cancer breast epithelial cells (MCF-7 cells) performed on tissue culture dishes using monoclonal antibodies against NGF. Calibration bar represents 100 mm. (b) Fluorescence confocal micrographs showing breast cancer cells (MCF-7) and NBECs treated with or without ionomycin. Cellular localization of NGF was assessed using secondary antibodies directed against anti-NGF coupled to Alexa 488. Ionomycin caused the disappearance of vesicles containing NGF. The calibration bar represents 10 mm

Article Snippet: They were then incubated overnight at 41C with a mouse monoclonal anti-human b-NGF antibody (R&D System), followed by 2 h at room temperature with biotinylated antimouse IgG antibodies diluted 1/250 and 2 h at room temperature with avidin/biotin–peroxidase complex diluted 1/200.

Techniques: Immunocytochemistry, Bioprocessing, Fluorescence

Journal: Oncogene

Article Title: Nerve growth factor overexpression and autocrine loop in breast cancer cells.

doi: 10.1038/sj.onc.1206805

Figure Lengend Snippet: Figure 5 NGF autocrine stimulation of breast cancer cell growth. (a) Time-dependent inhibition of basal breast cancer cell growth in a serum-free medium (&) with 1 mg/ml MAB-256 neutralizing anti- NGF (n) or with 10 nM K-252a ( ). Cells were counted after trypsinization with a hemocytometer. No modification of cellular growth was observed with control antibodies (not shown). (b) Dose effect of K-252a on MCF-7 cell growth and (c) dose effect of neutralizing anti-NGF. Cells were plated at 10 000/ml, and after 24 h of incubation in a serum-free medium, the indicated concentrations of anti-NGF and K-252a were added. Results presented in (a–c) represent the means7s.d. of five independent experiments

Article Snippet: They were then incubated overnight at 41C with a mouse monoclonal anti-human b-NGF antibody (R&D System), followed by 2 h at room temperature with biotinylated antimouse IgG antibodies diluted 1/250 and 2 h at room temperature with avidin/biotin–peroxidase complex diluted 1/200.

Techniques: Inhibition, Control, Incubation